Sterilization procedures for 2 incubators">CO2 incubators

Erscheingsdatum 
August 2018
Autoren 
Leonard Knegendorf, Holger Brill, Jörg Steinmann, Steffen Pahl, Eike Steinmann, Florian H. H. Brill
Bibliografische Daten 
Reviews in Medical Microbiology: August 16, 2018 - Volume Publish Ahead of Print - Issue - p, doi: 10.1097/MRM.0000000000000145, UNIVITED REVIEW ARTICLE: PDF Only, Copyright © Wolters Kluwer Health, Inc. All rights reserved.
Abstract 

Cell culture methods are utilized ubiquitously in science and pharmaceutical and biotech industries. Sterility is one crucial factor for maintenance of cells and creation of valid data from experiments. As cells are usually cultured in 2 incubators">CO2 incubators, those are one bottleneck in terms of sterility in a cell culture lab. This review gives an overview on the different sterilization procedures for 2 incubators">CO2 incubators on the market with emphasize to considerations from a practical point of view. It compares sterilization by dry heat, steam, gas and ultraviolet radiation in terms of validity and practicability in accordance with international standards and regulations and transfers literature consensus about these methods to 2 incubators">CO2 incubators. As conclusion, the authors give recommendations for a sterile working environment in line with good cell culture practice.