Virucidal activity of who-hand rub formulations against enveloped viruses including Zika, Ebola and emerging coronaviruses

Erscheingsdatum 
Juni 2017
Autoren 
Florian H. H. Brill, Anindya Siddharta, Stephanie Pfaender, Nathalie J. Vielle, Ronald Dijkman, Martina Friesland, Britta Becker, Jaewon Yang, Michael Engelmann, Daniel Todt, Marc P. Windisch, Joerg Steinmann, Jochen Steinmann, Stephan Becker, Marco P. Alves, Thomas Pietschmann, Markus Eickmann, Volker Thiel, Eike Steinmann
Bibliografische Daten 
Meeting abstracts from International Conference on Prevention & Infection Control (ICPIC 2017), Geneva, Switzerland. 20-23 June 2017; Antimicrobial Resistance & Infection Control2017 6(Suppl 3):P330, DOI: 10.1186/s13756-017-0201-4 - P330 - Hand hygiene: in-vitro to in-vivo
Abstract 

Introduction: To prevent viral transmission, hand hygiene is the most important measurement to reduce the spread of infections. The World Health Organization (WHO) published two alcohol-based formulations to be used in healthcare settings and outbreak situations. Inactivation efficacies of these products have not been determined against (re-) emerging viruses.

Objectives: In this study, we evaluated the virucidal activity of the WHO products in a comparative analysis against enveloped viruses. Methods: Virucidal activity studies were performed with a quantitative suspension test with 30 seconds exposure time. One part by volume of test virus suspension and one part by volume of the organic load were mixed with eight parts by volume of one of the two WHO formulations at different concentrations.

Results: Zika virus (ZIKV), Ebola virus (EBOV), Severe Acute Respiratory Syndrome coronavirus (SARS-CoV), Middle East Respiratory Syndrome coronavirus (MERS-CoV), bovine coronavirus (BCoV), hepatitis C virus (HCV), influenzavirus (H1N1) and Modified Vaccinia virus Ankara (MVA) served as test viruses. All viruses tested were highly susceptible to WHO formulations. Isopropanol-based WHO formulation II demonstrated a superior virucidal effect compared to ethanolcontaining WHO formulation I. Coronaviruses and ZIKV showed the highest susceptibility to both WHO formulations. Higher concentrations were required for complete inactivation of EBOV and HCV, H1N1 and MVA displayed the highest stability.

Conclusion: WHO alcohol-based formulations show a strong virucidal effect against emerging pathogens including ZIKV, EBOV, SARS- and MERS-CoV could be demonstrated implicating the usability of these WHO formulations in healthcare and outbreakassociated viral infections.

Disclosure of Interest
None Declared